# Multiple autoclaving of BioAmber parts

**URL:** <https://forum.formlabs.com/t/multiple-autoclaving-of-bioamber-parts/35666>\
**Category:** Materials\
**Created:** [February 13, 2023, 12:31pm UTC](https://forum.formlabs.com/t/multiple-autoclaving-of-bioamber-parts/35666 "2023-02-13T12:31:17Z")\
**Posts on this page:** 4\
**Page:** 1

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**Author:** ![SAP](https://avatars.discourse-cdn.com/v4/letter/s/3da27b/32.png) [@SAP](https://forum.formlabs.com/u/SAP)\
**Post date:** [February 13, 2023, 12:31pm UTC](https://forum.formlabs.com/t/multiple-autoclaving-of-bioamber-parts/35666/1 "2023-02-13T12:31:17Z")

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Hi, I am using 3B+ printed BioAmber parts for cell culture, but not in direct contact with the cells. I would like to reuse the parts and would like to find out if its possible to repeatetly autoclave these parts?  
If they are not suitable for repeated autoclaving, would repeated disinfection with ethanol affect the material properties or endurance?  
Thanks for any feedback.

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**Author:** ![Jennifer\_K](https://sea1.discourse-cdn.com/flex025/user_avatar/forum.formlabs.com/jennifer_k/32/18872_2.png) [@Jennifer\_K](https://forum.formlabs.com/u/Jennifer_K)\
**Post date:** [February 13, 2023, 3:23pm UTC](https://forum.formlabs.com/t/multiple-autoclaving-of-bioamber-parts/35666/2 "2023-02-13T15:23:38Z")

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Hello @SAP,

This is a great question on repeated autoclaving of parts. We have a results writeup on Biomed Amber sterilization and multiple sterilization and its effect on parts - please reach out to [Support](https://support.formlabs.com/s/contact-support?language=en_US) for a copy. This will help you make the call of whether multiple autoclaving cycles or just disinfecting with ethanol is best for you.

Thanks!  
-Jen

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**Author:** ![SAP](https://avatars.discourse-cdn.com/v4/letter/s/3da27b/32.png) [@SAP](https://forum.formlabs.com/u/SAP)\
**Post date:** [February 14, 2023, 4:14pm UTC](https://forum.formlabs.com/t/multiple-autoclaving-of-bioamber-parts/35666/3 "2023-02-14T16:14:02Z")

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Hi Jen,

Thank you very much for the quick and helpful answer 🙂

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**Author:** ![mwbarre1](https://sea1.discourse-cdn.com/flex025/user_avatar/forum.formlabs.com/mwbarre1/32/14449_2.png) [@mwbarre1](https://forum.formlabs.com/u/mwbarre1)\
**Post date:** [February 16, 2023, 3:42pm UTC](https://forum.formlabs.com/t/multiple-autoclaving-of-bioamber-parts/35666/4 "2023-02-16T15:42:51Z")

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We have autoclaved Surgical Guide materials for our cell culture needs. Repeated autoclaving is only possible for a few times before cracking appears. It’s tough material, that’s for sure. But Autoclaving is a rough process. We have also reduced our autoclave process temps to minimal and increased time for process.